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Antibodies

Cholesterol Oxidase Antibody

Product Sizes
2 ml
100-101-213-2ML
About this Product
SKU:
100-101-213
Additional Names:
choA|goat anti-Cholesterol Oxidase Antibody, choA antibody, choD antibody, choM antibody, choS antibody, COD antibody
Application:
ELISA, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
90 mg/ml
Extra Details:
Cholesterol Oxidase is a bifunctional enzyme that catalyzes the oxidation of the 3-beta-hydroxy group of cholesterol and the isomerization of the double bond of the resulting product. This protein is involved in the pathway cholesterol metabolism, which is part of Steroid metabolism.
Format:
Antiserum
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Cholesterol Oxidase [Streptomyces]
Physical State:
Lyophilized
Reactivities:
Bacteria
Shipping Conditions:
Ambient
Specificity:
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Cholesterol Oxidase [Microorganism]. Cross reactivity against Cholesterol Oxidase from other sources is unknown.
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Rockland Goat anti Cholesterol oxidase antibody (200-101-213 lot 6534) was used to detect purified cholesterol oxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified cholesterol oxidase contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with a 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System.