K48 PANEL (IQF)
Product Sizes
9.2 ug
£959.00
DU-0101-4800-9.2UG
About this Product
- SKU:
- DU-0101-4800
- Additional Names:
- HMG20;polyubiquitin-C
- Application:
- Cell-based/Functional Assay
- Conjugate:
- TRITC
- Extra Details:
- IQF DiUb Technology \nLifeSensors' diubiquitin substrates represent a new class of substrates for the continuous fluorescent measurement of true isopeptidase activity. The C-terminus of wild type ubiquitin is conjugated via an isopeptide bond to lysine 48 (K48) of a second ubiquitin molecule with the resultant diubiquitin forming an internally quenched fluorescent FRET pair (IQF). Each ubiquitin is labeled with a single molecule of either a fluorescent reporter (i.e. TAMRA) or a highly efficient quenching fluorophore. Cleavage of the IQF DiUb by deubiquitylases leads to separation of the fluorophore from quencher and subsequent increase in observed fluorescence. \n \nIQF DiUb Variants \nThere is a remarkable difference between K48- and K63-linked ubiquitin molecules in their three-dimensional structure, which accounts for their individual functions. Beyond differential linkages (e.g. K48, K63, K11), LifeSensors has created subpanels of IQF diubiquitin substrates within each linkage. Because each DUB is likely to recognize and cleave substrates with unique steric considerations, these subpanels vary in location of reporter fluorophore and quencher. It is recommended that each DUB be empirically evaluated against a panel of IQF DiUb substrates to select the the optimal fluorophore/quencher pairing.In order to rapidly determine which of these fluorophore/quencher pairs are optimal for your DUB and/or determined linkage specificity for your DUB, LifeSensors offers a panel of 6 substrates comprised of each K48-linked substrate available to date.
- Gene Details:
- UBC
- Protein Details:
- P0CG48
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- 2-8[o]C
- Supplier:
- LifeSensors
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
- Manufacturer's Data Sheet:https://lifesensors.com/product/du0101-k48-panel-iqf/