Thermolabile UNG (Uracil N-Glycosylase), Prevention of carry-over contaminations
Product Sizes
200 units
£235.00
PCR-353-200UNITS
About this Product
- SKU:
- PCR-353
- Additional Names:
- UNG, UDG
- Application:
- PCR
- Concentration:
- 1 unit/ul
- Extra Details:
- Thermolabile UNG is used in real-time PCR to prevent carry-over contamination of dU-containing DNA from previous reactions. Uracyl N-Glycosylase (UNG, UDG) catalyses the release of uracil from single and double stranded uracyl-containing DNA. The resulting abasic sites are susceptible to hydrolytic cleavage at elevated temperatures. An amount of 0.1 units UNG can completely destroy up to 200 ng dU-containing DNA in 2 min at 50°C. Recommended assay: Add 0.2 ul (0.2 units) UNG for each 50 ul of master mix and vortex thoroughly. The preparation of a master mix is crucial in quantitative PCR reactions to reduce pipetting errors. An UNG treatment of 2 min at 50°C at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template. UNG is easily heat-inactivated at temperatures above 65°C in the following initial denataration step of the PCR.
- Physical State:
- Liquid
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Jena Bioscience
- Type:
- Molecular Biology Reagents: PCR