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Antibodies

Anti-Fascin (clone 55K2) Antibody

Product Sizes
100 ul
FM2651-100UL
About this Product
SKU:
FM2651
Additional Names:
p55
Application:
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
55K2
Clonality:
Monoclonal
Extra Details:
Our Fascin (clone 55K2) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Fascin (clone 55K2) and is Protein A purified. It is great for use in WB, ICC, IHC.
Format:
Protein A Purified
Gene Details:
FSCN1
Host:
Mouse
Immunogen:
Clone (55K2) was generated from full-length human fascin purified from HeLa cells. This sequence has high homology to rat and mouse fascin.
Isotype:
IgG1
Molecular Weight:
55
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects a 55 kDa* band corresponding to fascin on SDS-PAGE immunoblots of human A431 and HeLa, as well as mouse C2C12 and rat PC12.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Monoclonal Antibody
Immunocytochemical labeling of Fascin in aldehyde fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Fascin (FM2651). The antibody was detected using goat anti-mouse DyLight® 594.
Western blot analysis of human HeLa cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Fascin (clone 55K2) (lanes 1 & 2) and anti-Fascin (Ser-39) (lanes 3 & 4).
Sea urchin embryos were injected with control miR-124 LNA inhibitor or miR-31 LNA inhibitor and immunolabeled with Fascin antibody (cat. FM2651, green) and tubulin antibody (magenta) followed by DAPI counterstain for DNA (blue). Image from publication CC-BY-4.0. PMID: 37398341
Embryos were immunolabeled with Fascin antibodies (cat. FM2651, 1:100) pre-adsorbed with lysate from bacteria expressing HA-tagged Fascin or human Rab35-GST. Embryos immunolabeled with pre-adsorbed Fascin antibodies have decreased Fascin (shown in green) compared to embryos immunolabeled with non-pre-adsorbed Fascin antibody. Image from publication CC-BY-4.0. PMID: 37033709