Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Anti-eEF2K (Ser-500), Phosphospecific Antibody

Product Sizes
100 ul
EP4451-100UL
About this Product
SKU:
EP4451
Additional Names:
eEF-2, eEF-2K, CaMK-III, eukaryotic elongation factor
Application:
Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Ser-500 - Phosphorylated
Extra Details:
Our eEF2K (Ser-500) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat eEF2K (Ser-500) and is antigen affinity purified. It is great for use in WB.
Format:
Antigen Affinity Purified
Gene Details:
EEF2K
Host:
Rabbit
Immunogen:
Phospho-eEF2K (Ser-500) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-500 in human eEF2K. This sequence is well conserved in rat and mouse eEF2K, and has low homology to other proteins.
Isotype:
IgG
Molecular Weight:
105
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects a 105 kDa* protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of human recombinant eEF2K autophosphorylated in the presence of Ca2+ and Calmodulin. This reactivity is removed after lambda phosphatase treatment. In addition, the antibody detects eEF2K after calyculin A treatment of mouse C2C12 cells, but not in control cells.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Recombinant human eEF2K untreated (lanes 1 & 4), auto-phosphorylated in the presence of Ca2+ and Calmodulin (lanes 2 & 5), and dephosphorylated with lambda phosphatase (lanes 3 & 6). The blots were probed with rabbit polyclonal anti-eEF2K (N-terminus) (lanes 1-3) or anti-eEF2K (Ser-500) (lanes 4-6). (Images provided by the laboratory of Dr. Kevin Dalby in the Dept. of Pharmacy at the University of Texas at Austin.)
Western blot image of C2C12 untreated (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 min. (lanes 2 & 4). The blot was probed with anti-eEF2K (N-terminus) (lanes 1 & 2) and anti-eEF2K (Ser-500) (lanes 3 & 4).