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Antibodies

Anti-D Delta1-Catenin (Tyr-228), Phosphospecific Antibody

Product Sizes
100 ul
CM3561-100UL
About this Product
SKU:
CM3561
Additional Names:
pp120 Src substrate, p120
Application:
Immunocytochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
M356
Clonality:
Monoclonal
Conjugate:
Tyr-228 - GOmAb/Phosphorylated
Extra Details:
Our D Delta1-Catenin (Tyr-228) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat D Delta1-Catenin (Tyr-228) and is Protein A purified. It is great for use in WB, ICC.
Format:
Protein A Purified
Gene Details:
CTNND1
Host:
Mouse
Immunogen:
Clone M356 was generated from a phospho-D Delta1-Catenin (Tyr-228) synthetic peptide containing amino acid residues around tyrosine 228 of human D Delta1-Catenin. This peptide sequence is highly conserved in rat and mouse D Delta1-Catenin.
Isotype:
IgG1
Molecular Weight:
110
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects a 110 kDa* protein corresponding to the molecular mass of D Delta1-Catenin on SDS-PAGE immunoblots of human A431 and HUVEC cells treated with pervanadate. This antibody does not detect this band after alkaline phosphatase treatment.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Monoclonal Antibody
Western blot analysis of D Delta1-Catenin phosphorylation in A431 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1,3,5,7,9). The blot was then treated with alkaline phosphatase (lanes 2,4,6,8,10). Blots were probed with anti-D Delta1-Catenin (a.a. 275-285) (lanes 1 & 2), anti-D Delta1-Catenin (Tyr-96) (lanes 3 & 4), anti-D Delta1-Catenin (Tyr-228) (lanes 5 & 6), anti-D Delta1-Catenin (Tyr-280) (lanes 7 & 8) or anti-D Delta1-Catenin (Tyr-302) (lanes 9 & 10).
Immunocytochemical labeling of D Delta1-Catenin in untreated (Top) or pervanadate-treated (bottom) A431 cells. The cells were labeled with mouse monoclonal D Delta1-Catenin (a.a. 275-285), D Delta1-Catenin (Tyr-228), D Delta1-Catenin (Tyr-280), or D Delta1-Catenin (Tyr-302) antibodies. The antibodies were detected using donkey anti-mouse secondary antibodies conjugated to Cy3.