Oligomeric Amyloid-beta, Human, Rat, ELISA assay-2 Plate Kit
Product Sizes
2 Plate Kit
£757.00
BEK-2215-2P-2PLATEKIT
About this Product
- SKU:
- BEK-2215-2P
- Additional Names:
- Beta-APP42; Beta-APP40; Beta-amyloid protein 42; Beta-amyloid protein 40; ABPP; APPI; Amyloid beta A4 protein; MOAB2; MOAB-2; Alzheimer's antibody; AB40; AB42; abeta.
- Application:
- ELISA
- Extra Details:
- The oligomeric form of Amyloid Beta peptide (Aβ, 1-42) has been closely linked to Alzheimer's Disease. Several ELISAs targeting Aβ have been developed; however, these ELISAs are known to cross-react with Amyloid Beta precursor protein (APP) and are poorly characterized against monomeric and oligomeric forms of the peptide. The Biosensis MOAB-2 antibody, developed by LaDu and co-workers (Youmans K. et al., 2012), has been shown to specifically detect Aβ, but not the precursor molecule APP. When utilized in ELISAs, the oligomeric form of Aβ peptide (o-Aβ) can be assayed independently of the other forms of the molecule when assayed with the MOAB-2 monoclonal antibody.
The Biosensis oligomeric Aβ ELISA kit is a sandwich ELISA that allows the preferential quantification of oligomeric Aβ peptides. This kit is exclusive to Biosensis and consists of a pre-coated mouse monoclonal anti-Aβ capture antibody (MOAB-2), a biotinylated MOAB-2 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of o-Aβ present in samples and protein standards. The purpose of this kit is the in vitro qualitative measurement of oligomeric Aβ peptide levels in brain extracts and CSF samples from both transgenic mice and humans relative to a known o-Aβ standard. The inclusion of a highly validated oligomeric standard results in a unique, ready-to-use ELISA kit.
This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. - Host:
- Human
- References:
- Youmans KL et al. <a class="newA" target="_blank" href='http://www.ncbi.nlm.nih.gov/pubmed/21219931'> (2011) J Neurosci Methods 196(1): 51-9</a><br>Youmans KL et al. <a class="newA" target="_blank" href='http://www.ncbi.nlm.nih.gov/pubmed/22423893'> (2012) Mol Neurodegener. 16;7:8</a><br>Tai ML et al. <a class="newA" target="_blank" href='http://www.ncbi.nlm.nih.gov/pubmed/23293020'> (2013) J Biol Chem. 288(8): 5914-26</a><br>Good pathology summary: <br>Viola KL, Klein WL. (2015) Amyloid _ oligomers in Alzheimer's disease pathogenesis, treatment, and diagnosis. <a class="newA" target="_blank" href='http://www.ncbi.nlm.nih.gov/pubmed/25604547'> Acta Neuropathol. 2015 Feb;129(2):183-206</a>
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- 2-8°C (on cold packs)
- Supplier:
- Biosensis