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Antibodies

anti-FAP / Fibroblast activation protein antibody

Product Sizes
100 ul
ARG66701-100UL
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
ARG66701
Additional Names:
Fibroblast activation protein alpha; SIMP; Seprase; Integral membrane serine protease; Surface-expressed protease; EC 3.4.21.26; Dipeptidyl peptidase FAP; 170 kDa melanoma membrane-bound gelatinase; EC 3.4.14.5; APCE; Serine integral membrane protease; DPPIV; EC 3.4.21.-; FAPalpha; Gelatine degradation protease FAP; Post-proline cleaving enzyme; Prolyl endopeptidase FAP; FAPA
Application:
IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol.
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]
Host:
Rabbit
Immunogen:
KLH-conjugated synthetic peptide within the center region of Human FAP / Fibroblast activation protein.
Isotype:
IgG
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Polyclonal Antibody
Immunofluorescence: Formalin-fixed HeLa cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained with ARG66701 anti-FAP / Fibroblast activation protein antibody (red) in 3% BSA-PBS and incubated overnight at 4°C. DAPI (blue) for nuclear staining.
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human prostate cancer tissue. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The tissue section was stained with ARG66701 anti-FAP / Fibroblast activation protein antibody at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
Western blot: HeLa, MCF7, NIH/3T3, Mouse kidney and Rat liver lysates stained with ARG66701 anti-FAP / Fibroblast activation protein antibody.