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Antibodies

anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody

Product Sizes
100 ug
£818.00
ARG43732-100UG
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
ARG43732
Additional Names:
SARS-CoV-2 (COVID-19) Spike Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein
Application:
ELISA, Western Blot
Buffer:
PBS and 0.02% Sodium azide.
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
In January of 2021 a new lineage of SARS-CoV-2, known as P.1 and named as Gamma variant, was discovered in Japan and later spread in Brazil. It is considered as VOC (variant of concern). This variant carries 10 mutations in spike protein, including N501Y, E484K and K417T in RBD, which can increase the affinity to the human ACE2 receptor. Enhanced transmission of the Gamma variant (P.1 lineage) was observed globally, which is 3.5 times more contagious as the original one. The Gamma variant affects the effectiveness of COVID19 vaccine and is resistant to neutralization to some extent due to the immune escape E484K mutation.
Host:
Rabbit
Isotype:
IgG
Purification:
Affinity Purified
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Polyclonal Antibody
Direct ELISA: 100ul of SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529) at a concentration of 1 ug/mL, incubated in a 96-well plate at 4 °C for overnight. The coated plate were incubated with 1 ug/mL or 0.2 ug/mL of ARG43732 anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody at RT for 1 hr. ARG43732 anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody detects Gamma Variant spike S1 protein only.
Direct ELISA: SARS-CoV-2 Spike S1 WT protein (blue) or gamma variant (P.1) (red) was coated on the plate and ARG43732 anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody was used as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. And ARG43732 anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody did not react to SARS-CoV-2 Spike S1 WT protein.
Western blot: 10 ug of SARS-CoV-2 Spike S1 WT protein (right) or gamma variant (left) expression plasmid transfected 293 (right) cell lysate stained with ARG43732 anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody for 1 hour incubation at RT in 5% NFDM/TBST, at 1 ug/ml dilution.