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Antibodies

anti-SARS-CoV-2 Spike protein (S2) antibody

Product Sizes
100 ug
£796.00
ARG43282-100UG
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
ARG43282
Application:
IHC-Paraffin, Western Blot
Buffer:
PBS and 0.02% Sodium azide.
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.
Host:
Rabbit
Isotype:
IgG
Purification:
Affinity Purified
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Polyclonal Antibody
Immunohistochemistry: Paraffin-embedded COVID-19 patient lung tissue. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG43282 anti-SARS-CoV-2 Spike protein (S2) antibody at 0.1 ug/ml dilution, overnight at 4°C.
Western blot: 30 ng of SARS-CoV-2 Spike S2 recombinant protein stained with ARG43282 anti-SARS-CoV-2 Spike protein (S2) antibody at 1 ug/ml dilution, 1 hour incubation at RT in 5% NFDM/TBST.
Western blot: Spike transfected 293 cells. 10 ug of cell lysate stained with ARG43282 anti-SARS-CoV-2 Spike protein (S2) antibody at 1 ug/ml dilution, 1 hour incubation at RT in 5% NFDM/TBST.