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Antibodies

anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody

Product Sizes
50 ul
£525.00
ARG11154-50UL
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
ARG11154
Application:
Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
PBS, 5 mM Sodium azide and 50% Glycerol.
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Host:
Rabbit
Isotype:
IgG
Purification:
Purified
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Polyclonal Antibody
Immunofluorescence: HEK293 cells transfected with pCI-Neo-Mod vector including DNA encoding the SARS-CoV-2 Spike protein ACE2 binding domain (aa. 308-541 in the Spike protein sequence in Wuhan-Hu-1). This region was used as the immunogen for development of ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody. HEK293 cells stained with ARG11154 (red) at 1:3000 dilution, and co-stained with anti-beta Tubulin antibody (green) at 1:5000 dilution. Hoechst (blue) for nuclear staining.
Immunofluorescence: Vero cells infected with SARS-CoV-2 virus. Cells were stained with ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody (red). DAPI (blue) for nuclear staining.
Western blot: 1) Non-transfected HEK293 cells, 2) HEK293 cells transfected with pCI-Neo-Mod vector containing the SARS-CoV-2 Spike protein ACE2 binding domain cDNA, and 3) HEK293 cells transfected with pCI-Neo-GFP vector containing the SARS-CoV-2 Spike protein ACE2 binding domain cDNA. Cell lysates were stained with ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody (green) at 1:2000 dilution. The same blot was simultaneously stained with anti-beta Tubulin antibody (red) at 1:5000 dilution.
Western blot: Full-length recombinant SARS-CoV-2 Spike protein expressed in HEK293 cells. Loading of 1 ug (left) or 100 ng (right). The blots were stained with ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody.