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BL21 (DE3) Chemically Competent E. coli Cells | Recombinant Proteins Production

Product Sizes
10 X 0.1 mL/tube
BL21-201-10X0.1ML/TUBE
10 x 0.1 ml
BL21-201-10X0.1ML
About this Product
SKU:
BL21-201
Application:
Protein Engineering
Extra Details:
BL21(DE3) chemically competent E.coli cells are suitable for transformation, high-level expression and production of recombinant proteins in bacterial systems. The strain lacks the lon and ompT proteases thus promoting stability of recombinant proteins. BL21(DE3) strain carries a copy of the T7 RNA polymerase under control of the IPTG inducible lacUV5 promoter, and as a result, is ideal for controlled expression of both E. coli and T7 promoter driven constructs. Compatible Expression Vectors/Promoters: Ptac, Ptrc, Plac, PlacUV5, ParaBAD, PrhaBAD, T7 The transformation efficiency is ≥ 1x107 cfu/ug pUC19 plasmid DNA. BL21 (DE3) chemically competent E. coli cells are supplied as a pack of 10 ready-to-use 100 ul/tube aliquots. Electrocompetent BL21 (DE3) E.coli cells are also available. Bulk quantities and custom packaging are available at very competitive prices for all Competent Cells. Catalog # BL21-201 E. coli genotype: fhuA2 [lon] ompT gal (L Lambda DE3) [dcm] D DeltahsdS. L Lambda DE3 = L Lambda sBamHIo D DeltaEcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 D Deltanin5 Application Ideal for expressing proteins in E.coli. BL21(DE3) cells carry the lambda DE3 lysogen for expression from vectors utilizing the T7 promoter.
Shipping Conditions:
Blue Ice
Storage Conditions:
-70[o]C
Supplier:
Amid Biosciences
Type:
Cells: Non-Human Cells