TR-FRET
2BScientific offers an extensive breadth and depth of reagents to support research endevours. In doing so, we feature a diverse portfolio encompassing a comprehensive workflow for TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) assays. TR-FRET is a powerful technology that allows researchers to investigate molecular interactions and biomolecular processes with remarkable precision, making it an invaluable tool in advancing scientific research.
Assay Kits for TR-FRET:
Using assay kits simplifies the experimental process by providing all necessary components in a single package. This convenience saves time and effort in procuring and preparing individual reagents. Additionally, these kits offer standardised protocols and optimised reagents, ensuring consistency and reliability across experiments.
Buffers & General Consumables:
Buffers play a critical role in TR-FRET assays by maintaining the required pH, ionic strength, and
environmental conditions. They create an ideal environment for accuracte measurements and help prevent non-specific binding of molecules. This ensures the specificity and precision of TR-FRET results.
Conjugated Proteins:
Conjugated Proteins labelled with fluorescent or luminescent markers, enable precise and specific
detection of protein-protein interactions or binding events. Their use enhances the sensitivity of TR-FRET assays, as fluorescence is emitted only when the labels are in close proximity. This real-time monitoring capability is invaluable for studying dynamic molecular processes.
Tagged Secondary Antibodies:
Tagged secondary antibodies,labelled with fluorescent or luminescent tags, provide versatility in TR-
FRET assays. They can selectively bind to target molecules, allowing researchers to develop assays for a wide range of biomolecules. This versatility enables the study of various biological interactions, localisation within samples, and quantitative analysis, making TR-FRET assays adaptable to diverse research needs.
BioAuxilium offers a diverse array of TR-FRET products, and are highly skilled 
in designing, developing, and producing assay kits and reagents for popular technology platforms. This comprehensive range includes THUNDER™ TR-FRET Cell Signalling Assay Kits, THUNDER™ TR-FRET Biomarker Assay Kits and THUNDER™ TR-FRET cAMP Assay Kit, all of which are invaluable for advancing research in this field and are available from 2BScientific.
Poly-Dtech presents a comprehensive range of lanthanide-based fluorophore-conjugated streptavidin products, including their Bright-Dtech series. These specialised reagents are optimised for TR-FRET assays, enabling researchers to capture and detect biotinylated molecules for diverse applications, such as protein-protein interaction studies, biomolecular assays, and drug discovery research. These products offer advantages like fast and wash-free protocols, minimal background noise, low sample consumption, and significantly improved limit of detection (LOD).