Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

4-Hydroxynonenal Antibody, Clone 12F7: FITC

Product Sizes
100 ug
SMC-511D-FITC-100UG
About this Product
SKU:
SMC-511D-FITC
Additional Names:
4-Hydroxynonenal Antibody, 4-Hydroxy Nonenal (4-HNE) Antibody, 4-Hydroxy Nonenal Antibody, 4-HNE Antibody, 4-hydroxy Hexenal Antibody, HNE Antibody, 4-hydroxy-2-nonenal Antibody
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
12F7
Clonality:
Monoclonal
Conjugate:
FITC
Concentration:
1 mg/ml
Extra Details:
4-Hydroxy-2-nonenol (4-HNE) is an unsaturated aldehyde derived from lipid peroxidation. 4-HNE is an electrophile and reacts with protein nucleophiles such as cysteine, histine, and lysine (1). Low levels of 4-HNE promote cell survival via cellular antioxidant induction whereas higher levels lead to autophagy, apoptosis, and ultimately necrosis. 4-HNE has been linked to Alzheimer's disease, Parkinson's disease, cancer, cardiovascular diseases, diabetes, and liver disease.
Host:
Mouse
Immunogen:
Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
Isotype:
IgG1
Purification:
Protein G Purified
Reactivities:
Wide/Non-species Specific
Shipping Conditions:
Blue Ice
Specificity:
Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Storage Conditions:
See Manual
Supplier:
StressMarq Biosciences
Type:
Antibody: Monoclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody, Clone 12F7 (SMC-511). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (SMC-511) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) 4-Hydroxynonenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Western Blot analysis of 4-hydroxy-nonenal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-nonenal protein using Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody, Clone 12F7 (SMC-511). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: 4-hydroxyl nonenal-BSA (0.5 ug). Lane 4: 4-hydroxy nonenal-BSA (2.0 ug). Lane 5: 4-hydroxy-2-hexenal (0.5 ug). Lane 6: 4-hydroxy-2-hexenal (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody (SMC-511) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.