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Antibodies

CavBeta2 Antibody, Clone N8b/1: APC

Product Sizes
100 ug
SMC-332D-APC-100UG
About this Product
SKU:
SMC-332D-APC
Additional Names:
Cacnlb2 Antibody, cacnb2 Antibody, Voltage-dependent L-type calcium channel subunit beta-2 Antibody, CAB2 Antibody
Application:
Antibody Microarray, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
N8b/1 (Formerly sold as S8B-1)
Clonality:
Monoclonal
Conjugate:
APC
Concentration:
1 mg/ml
Extra Details:
Cav Beat subunits are involved in the transport of the pore forming alpha1 subunit to the plasma membrane (1). They also shield an ER Retention signal on the alpha1 subunit, thereby guiding the pore-forming subunit to the target membrane (2, 3). They also determine the biophysical properties of the calcium channel (3).
Host:
Mouse
Immunogen:
Synthetic peptide amino acids 189-205 of rat CavBeta2
Isotype:
IgG1
Purification:
Protein G Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
Detects ~78 kDa. No cross reactivity against CavB Beta1, CavB Beta3, CavB Beta4.
Storage Conditions:
See Manual
Supplier:
StressMarq Biosciences
Type:
Antibody: Monoclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav beta 2 Monoclonal Antibody, Clone S8B-1 (SMC-332). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav beta 2 Monoclonal Antibody (SMC-332) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav beta 2 Antibody (D) Composite.
Immunohistochemistry analysis using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (SMC-332). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (SMC-332) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All nuclei. Some nuclei also staining higher up in epidermis.
Western Blot analysis of Human Cell line lysates showing detection of Cav Beta2 Calcium Channel protein using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (SMC-332). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (SMC-332) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (SMC-332). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (SMC-332) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive. Bright dottiness located throughout cytoplasm and in nuclei.