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0.5 M EDTA pH 8.0

Product Sizes
100 ml
MB-014-100ML
About this Product
SKU:
MB-014
Additional Names:
Ethylenediaminetetraacetic acid, EDTA
Application:
Flow Cytometry
Buffer:
See application note.
CE/IVD:
RUO
Concentration:
0.5 M
Extra Details:
EDTA is widely used for scavenging metal ion in biochemistry and molecular biology. Ion depletion is commonly used to deactivate metal-dependent enzymes, either as an assay for their reactivity or to suppress damage to DNA or proteins. In tissue culture EDTA is used as a chelating agent that binds to calcium and prevents joining of cadherins between cells, preventing clumping of cells grown in liquid suspension, or detaching adherent cells for passaging. EDTA is also known to inhibit a range of metallopeptidases, the method of inhibition occurs via the chelation of the metal ion required for catalytic activity.
Physical State:
Liquid
Shipping Conditions:
Ambient
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Buffers & General Consumables: Others
0.5 M EDTA pH 8.0
Systems-level analysis of immune development in human newborns. (A) Study overview. (B) Monocyte abundance analyzed by mass cytometry and IFNG Gamma and IL1RA measured by Olink assays in longitudinal blood samples (n = 858) from 208 individual children and binned by sampling day of life. Boxplots are colored by mean rank. CB, cord blood. (C) Blood mass cytometry analyses of memory Tregs. pDC, plasmacytoid DC. (D) Blood G GammaD DeltaT cell abundance and subset of G GammaD DeltaT cells expressing CD161 and plasma IL-17A. (E) Representative fluorescence-activated cell sorting (FACS) plots of CD38+CD62L-CD4+ T cells sorted at postnatal days 0, 4, 29, and 76 from newborn peripheral blood mononuclear cells (PBMCs) and subjected to bulk mRNA sequencing (mRNA-seq). (F) Gene set enrichment analysis showing the top enriched hallmark pathways in mucosal-specific versus total memory CD4+ T cells. Figure 1. PMID: 34143954.
Immune Cell Proportions in COVID-19. Proportion of 57 white blood cell populations determined by mass cytometry from acute to recovery phase of COVID-19 patients (n = 35 individuals). Loess smoothing in orange. Figure 3. PMID: 32838342.