Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Assay Kits & ELISAs

DyLight[TM] Multiplex 488/800 Duo Western Blot Kit

Product Sizes
1 kit
KFA015-1KIT
About this Product
SKU:
KFA015
Additional Names:
Fluorescent western blotting, Multiplex western blotting, multi-color western blotting, fluorescent labelled antibodies, fluorescent imaging, DyLights, dyLight conjugates
Application:
Western Blot
CE/IVD:
RUO
Extra Details:
The DyLight™ multiplex 488/800 Duo fluorescent Western blotting kit is suited for simultaneous detection and quantification of specific protein populations in a biological sample. Using a combination of two antibodies selected for minimal cross reactivity, fluorescent detection method enables simultaneous quantitative analysis of multiple proteins within the same sample on the same blot. The DyLight™ multiplex 488/800 Duo Western blot kit contain all the necessary components that are optimized for the simultaneous detection of multiple proteins on the same blot using DyLight™-dye labeled secondary antibodies that are visualized in different fluorescence channels (488/800). The kit also includes blocking buffer, wash buffer, pre-stained protein standard and an incubation box for convenience and ultimate performance with minimal or no optimization. The fluorescent dyes such as DyLights™ when conjugated to secondary antibodies, offer a variety of benefits over traditional detection methods such as colorimetric and chemiluminescent detection. Multiplex detection using the correct lighting and filter conditions, enables the quantitation of multiple proteins and eliminates the need to strip and reprobe. Other benefits of fluorescent Western blotting include increased sensitivity, excellent signal stability over time as well as precise quantitative analysis with broader dynamic range and high linearity. Due to their exceptional photostability, DyLight™ dye conjugates can be archived and visualized several times without a decrease in signal.
Shipping Conditions:
Blue Ice
Specificity:
The DyLight conjugated secondary antibodies were prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit or Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit or Mouse IgG, and Rabbit or Mouse Serum. No reaction was
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Assay Kits: Western Blot
Simultaneous detection of A Alpha-tubulin and GFP on a single blot using Rockland DyLight™-labeled secondary antibody conjugates. Protein lysates from HeLa (lane 1), PC12 (lane 2) and K562 (lane 3) cells and 100ng GFP protein (Lane 4) were run on a gel. The cell lysates were spiked with 25ng, 50ng and 75ng GFP protein. Probing of cell lysates and GFP with mouse anti-A Alpha-tubulin and chicken anti-GFP antibodies followed by DyLight™ 649 goat anti-mouse IgG (pseudocolored green) and DyLight™ 800 goat anti-chicken IgG (red) conjugates, and then imaged using Syngene G:BOX Imaging System resulted in comparable patterns of detection. Lane 5: Rockland Opal Prestained Protein Standard 10-245kDa.
Simultaneous detection of three proteins on a single blot using Rockland DyLight™-labeled secondary antibody conjugates. Protein lysates from HeLa (Lane 1), PC12 (Lane 2) and K562 (lane 3) cells and 50ng GFP protein (Lane 4) were run on a gel. The cell lysates were spiked with 50ng, 75ng and 150ng GFP protein. Probing of cell lysates and GFP with anti-A Alpha-tubulin (mouse), anti-B Beta-actin (rabbit), and anti-GFP (chicken) followed by DyLight™ 649 goat anti-mouse IgG (red), DyLight™ 800 goat anti-rabbit IgG (pseudocolored aqua) and DyLight™ 488 goat anti-chicken IgG (pseudocolored green) conjugates, and imaged using Syngene G:BOX Imaging System resulted in comparable patterns of detection.