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Assay Kits & ELISAs

E. coli HCP Chemiluminescent Western Blot Kit

Product Sizes
1 kit
KCAJ07-1KIT
About this Product
SKU:
KCAJ07
Additional Names:
Western Blotting Kit, Chemiluminescent Kit, Peroxidase Kit, Immunoblotting Kit, HCP, Host Cell Protein detection, HCP contaminants detection, ECP, ECP detection, ECP contaminants
Application:
Western Blot
CE/IVD:
RUO
Extra Details:
Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. The antibody provided in this kit recognizes high and low molecular weight protein fractions from E.coli. The Anti-Host Cell Proteins Antibody provided in this kit detects over 300 E.coli proteins and is suitable for western blotting. This antibody has been validated by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
Format:
Chemiluminescent
Immunogen:
Anti-Host Cell Protein Antibody (p/n 100-401-J07) was prepared blending antibodies that have been separately developed by repeated immunizations in rabbit with a mixture of high molecular weight (HMW) and a mixture of low molecular weight (LMW) proteins from E.Coli (BL21).
Reactivities:
Bacteria
Shipping Conditions:
Blue Ice
Specificity:
This Chemiluminescent Western Blot Kit allows for the detection of E.coli host cell proteins present in samples provided by the user. Included in the kit along with Rabbit Anti-Host Cell Protein antibody is a positive protein control, Goat Anti-Rabbit IgG HRP Antibody, and Blocking Buffer.
Storage Conditions:
See Manual
Supplier:
Rockland Inc
Type:
Assay Kits: Western Blot