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Assay Kits & ELISAs

DYKDDDDK (FLAG[R]) Chemiluminescent Western Blot Kit

Product Sizes
1 kit
KCA383-1KIT
About this Product
SKU:
KCA383
Additional Names:
Flag Western Blot, Western Blotting Kit, Chemiluminescent Flag Kit, Immunoblotting Kit, FLAG®, HRP Anti FLAG, DDK, FLAG, FLAG antibody, anti-DDK, DDK antibody
Application:
Western Blot
CE/IVD:
RUO
Extra Details:
The FLAG® epitope is a small but highly immunogenic peptide DYKDDDDK (N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C) which allows fusion proteins to retain their original conformation and function. The hydrophilic character of this sequence increases the likelihood that it will be located on the surface of FLAG® -tagged recombinant proteins for reaction with anti-DYKDDDDK (FLAG®) antibodies. The anti-DYKDDDDK (FLAG®) antibody included in this Western blot kit detects FLAG® -tagged recombinant proteins fused with FLAG® tag either at the amino-terminus or carboxy-terminus of the protein.
Format:
Chemiluminescent
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Blue Ice
Specificity:
This Chemiluminescent Western Blot Kit allows for the detection of FLAG® -tagged recombinant protein present in cell lysates provided by the user.
Storage Conditions:
See Manual
Supplier:
Rockland Inc
Type:
Assay Kits: Western Blot
Western Blot. Anti-DYKDDDDK (FLAG®) Chemiluminescent Kit for Western blot was used to detect known amounts of bacterial alkaline phosphatase (BAP) protein containing the FLAG® epitope tag. N-terminal linked and C-terminal linked BAP were diluted into a HeLa whole cell lysates. After separation by SDS-PAGE lysates were transferred to a membrane which was blocked with 5% BLOTTO (p/n B501-0500) in TTBS for 1 h at 4 °C. The blot was probed with Anti-DYKDDDDK (FLAG®) followed by HRP anti-Mouse IgG (p/n 610-103-121) at 1:10,000 dilution in blocking buffer for 1 h at 4°C.
Flow Diagram for Chemiluminescent Western Blot Procedure
Rockland Chemiluminescent Western Blot Kit for DYKDDDDK (FLAG®) has reagents to run up to 30 mini blots at 7.5 x 8 cm2
This product is assembled as a kit. See attached protocol or CofA for further details.