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Antibodies

Ubiquitin Hydrolase (UCHL1) Antibody

Product Sizes
100 ul
900-301-E52-100UL
About this Product
SKU:
900-301-E52
Additional Names:
mouse anti-UCHL1 Antibody, mouse anti-Ubiquitin Hydrolase Antibody, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1|UCHL1
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
BH7
Clonality:
Monoclonal
Extra Details:
UCHL1 antibody recognizes Ubiquitin C-terminal hydrolase 1 (UCHL1) which is also known as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5. It was later found that ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein. The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Genetic knockout of UCHL1 in mice results in a motor neuron degeneration similar to the spontaneous gracile axonal dystrophy (gad) mutant mice. Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease. Since UCHL1 is heavily expressed in neurons, it is released in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker. Therefore, UCHL1 monoclonal antibody is ideal for investigators involved in Neuroscience.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Mouse
Immunogen:
Anti-UCHL1 Monoclonal Antibody was produced in mouse by repeated immunizations with recombinant full length human UCHL1 extensively purified from E. coli.
Isotype:
IgG1
Physical State:
Liquid
Reactivities:
Bovine, Human, Mouse, Rat
Shipping Conditions:
Dry Ice
Specificity:
Anti-UCHL1 antibody is directed against human UCHL1 . The antibody is protein G purified from concentrated clarified cell culture supernate. Reactivity is expected against human, bovine, mouse, and rat sources. Cross reactivity from other sources has not been determined. However, expect reactivity with UCHL1 from most mammalian sources based on sequence homology.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Monoclonal Antibody
Immunofluorescence Microscopy of Mouse anti-Ubiquitin Hydrolase antibody. Tissue: Rat Spinal Chord. Fixation: 0.5% PFA Antigen retrieval: not required. Primary antibody: Ubiquitin Hydrolase antibody at 1:500 for 1 h at RT. Secondary antibody: Fluorescein mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: Ubiquitin Hydrolase fills the cytoplasma of their perikarva and dendrites. Staining: Ubiquitin Hydrolase as red fluorescent signal and NF-H with green fluorescent signal.
Western Blot of Mouse anti-Ubiquitin Hydrolase antibody. Lane 1: rat hippocampal homogenate. Lane 2: none. Load: 10 ug per lane. Primary antibody: Ubiquitin Hydrolase antibody at 1:5,000 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 24 kDa for Ubiquitin Hydrolase. Other band(s): none.