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Antibodies

Chicken IgM (mu chain) Secondary Antibody

Product Sizes
1 mg
603-101-007-1MG
About this Product
SKU:
603-101-007
Additional Names:
goat anti-Chicken IgM (mu chain) Antibody, goat anti-Chicken IgM mu chain Antibody
Application:
Cell-based/Functional Assay, ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.1 mg/ml
Extra Details:
Anti-Chicken IgM antibody specifically detects chicken IgM. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Chicken IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Chicken IgM whole molecule
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Avian
Shipping Conditions:
Blue Ice
Specificity:
Chicken Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgM and Chicken Serum. No reaction was observed against other Chicken heavy or li
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
SDS-Page of Goat anti-Chicken IgM (Mu Chain) antibody. Lane 1: Gt-a-Chicken IgM Antibody - Non-Reduced. Lane 2: Gt-a-Chicken IgM Antibody - Reduced. Load: 1.0 ug per lane. Predicted/Observed size: 50 kDa, 25 kDa for Reduced. Other band(s): none.
Western Blot of Anti-Chicken IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated (p/n 603-103-007). Lane M: 3 ul Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG F(ab) Fragment (p/n 003-0105). Lane 4: Chicken IgM Whole Molecule (p/n 003-0107). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated (p/n 603-103-007) 1:30,000 for 60 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.