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Antibodies

VSV-G Epitope Tag Antibody Dylight[TM] 488 Conjugated

Product Sizes
100 ug
600-441-386-100UG
About this Product
SKU:
600-441-386
Additional Names:
Rabbit Anti-VSV-G Epitope Tag DyLight 488™ Conjugated Antibody, Rabbit Anti VSV-G Epitope Tag DyLight 488™ Conjugated Antibody, Rabbit Anti-VSV-G Tag Antibody DyLight 488™ Conjugation
Application:
FLISA, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
DyLight™ 488
Concentration:
1 mg/ml
Extra Details:
In order to improve expression levels, solubility, folding, purification and detection of recombinant proteins, a very common strategy is the fusion of peptides or proteins also known as "tags", to the target protein. Because these tags are entities with known sequences and well characterized physicochemical properties, they are an essential tool in molecular biology that facilitates expression and purification of recombinant proteins. Because fusion tags constitute themselves antigenic epitopes for which antibodies can be developed they particularly useful for specific detection of the target protein. This Anti-VSV-G Epitope Tag Antibody generated in rabbit is conjugated to DyLight™488.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 501-511 (Y-T-D-I-E-M-N-R-L-G-K) of vesicular stomatitis virus glycoprotein (VSV-G) conjugated to KLH using maleimide.
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Ambient
Specificity:
This affinity purified antibody is directed against the VSV-G epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the VSV-G epitope tag (Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Tagged Antibodies
Confocal microscopy using Anti-VSV-G Epitope Tag Antibody DyLight™ 488 Conjugated. Surface localization of chimeric receptors. Confocal microscopy images of HEK293 cells expressing chimeric receptors with induction or reduction of CRE, SRE-.L and SRE reporters detected using an antibody directed against the extracellular N-terminal VSV-G epitope. Scale bars are 10 um. A total of 20,000 cells/well were seeded in 96-well plates and transfected with 100 to 150 ng receptor vector. After 48 h of transfection, the cells were washed with PBS and fixed with 4% PFA. After fixation, the cells were washed, blocked with 1% BSA in PBS, and incubated with antibodies against the N-terminal VSV-G epitope (DyLight™ 488 conjugated antibody, 600-441-386, Rockland, 1:500 final dilution in blocking buffer). For confocal microscopy, cells were washed and covered with mounting medium. Pictures of individual cells were recorded on an inverted confocal microscope (LSM 700, Zeiss, x63 objective, excitation and emission wavelengths were 488 and 500-700 nm, respectively.). Figure S6. PMID: 29769519.
DyLight™ dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. DyLight™ dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods. This image shows anti-histone detection using a DyLight™ 488 conjugate (green). Anti-Tubulin was detected using a DyLight™ 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).
Properties of Dylight™ Conjugates.