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Antibodies

ACINUS Antibody

Product Sizes
100 ug
600-401-X63-100UG
About this Product
SKU:
600-401-X63
Additional Names:
ACIN1|Acinus Antibody, ACN, ACINUS, fSAP152, KIAA0670, Apoptotic chromatin condensation inducer in the nucleus, Acinus
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE. A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS'.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-Acinus antibody was prepared from whole rabbit serum produced by repeated immunizations with a 35 amino acid peptide near the internal region of human Acinus.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
Anti-Acinus Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with Acinus from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence Microscopy of Acinus antibody. Tissue: K562 cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Acinus antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Acinus is nuclear. Staining: Acinus as green fluorescent signal.
Immunocytochemistry of Acinus antibody. Cells: K562 cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Acinus antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Acinus is nuclear. Staining: Acinus as precipitated brown signal with hematoxylin blue nuclear counterstain.
Western Blot of Acinus antibody. Lane 1: K562 whole cell lysate. Load: 35 ug per lane. Primary antibody: Acinus antibody at 1 ug/mL for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 151.9 kDa, 210 kDa for Acinus.