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Antibodies

DRAM Antibody

Product Sizes
100 ug
600-401-AX9-100UG
About this Product
SKU:
600-401-AX9
Additional Names:
DRAM Antibody, DRAM, DRAM, DNA damage-regulated autophagy modulator protein 1, Damage-regulated autophagy modulator|DRAM1
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-DRAM antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide from near the C-terminus of human DRAM.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Dry Ice
Specificity:
Anti-DRAM Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with DRAM from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of DRAM antibody. Lane A: 293 cell lysate at 0.5 ug/mL. Lane B: 293 cell lysate at 1 ug/mL. Load: 35 ug per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 26.2 kDa, ~29 kDa for DRAM. Other band(s): DRAM splice variants and isoforms.
Immunofluorescence Microscopy of DRAM antibody. Tissue: Human liver cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: DRAM antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: DRAM as red fluorescent signal.
Immunohistochemistry of DRAM antibody. Tissue: Human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: DRAM antibody at 2.5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: DRAM is cytoplasmic. Staining: DRAM as precipitated red signal with hematoxylin purple nuclear counterstain.
Immunofluorescence Microscopy of DRAM antibody. Tissue: Human Liver cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: DRAM antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: DRAM as red fluorescent signal.
Immunohistochemistry of DRAM antibody. Tissue: Human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: DRAM antibody at 2.5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: DRAM is cytoplasmic. Staining: DRAM as precipitated red signal with hematoxylin purple nuclear counterstain.