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Antibodies

Clusterin Antibody

Product Sizes
100 ug
600-401-AM0-100UG
About this Product
SKU:
600-401-AM0
Additional Names:
CLU|Clusterin Antibody, CLI, AAG4, APOJ, CLU1, CLU2, KUB1, SGP2, APO-J, SGP-2, SP-40, TRPM2, TRPM-2, NA1/NA2, CLI, Clusterin, Aging-associated gene 4 protein, Apo-J
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Clusterin, also known as Apolipoprotein J (ApoJ), is a ubiquitous multifunctional glycoprotein that can interact with a broad spectrum of molecules such as complement components, various receptors, and the Alzheimer's b-amyloid peptide. Clusterin expression is increased in Alzheimer's disease brain tissue and clusterin-immunoreactive amyloid plaques are found associated with phospho-tau-positive dystrophic neurites and it has been suggested that clusterin facilitates the conversion of diffuse b-amyloid deposits into amyloid and enhances tau phosphorylation in neurites around these plaques. Other reports show that clusterin expression is decreased in proliferating cells and is upregulated in quiescent and senescent cells, suggesting that it may also play a role in aging and tumorigenesis suppression. Clusterin exists in at least two distinct isoforms.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-Clusterin antibody was raised recombinant human Clusterin isoform 1.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
Anti-Clusterin Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with Clusterin from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Clusterin antibody in human brain tissue lysate. Lane A: Clusterin antibody at 0.5 ug/mL. Lane B: Clusterin antibody at 1 ug/mL. Load: 35 ug per lane. Primary antibody: Clusterin antibody at designated concentrations for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 52 kDa, 58 kDa for Clusterin. Other band(s): Clusterin splice variants and isoforms.
Immunohistochemistry of Clusterin antibody. Tissue: human brain tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Clusterin antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Clusterin is located in the cytoplasm, cytoplasmic vesicle, endoplasmic reticulum, membrane, microsome, mitochondrion, nucleus, and is secreted. Staining: Clusterin is stained with hematoxylin purple nuclear counterstain.