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Antibodies

Alpha Internexin Antibody

Product Sizes
100 ul
200-901-D04-100UL
About this Product
SKU:
200-901-D04
Additional Names:
Ina|internexin, NF-66, NEF5, Alpha-internexin, Alpha-Inx, Ina, Inexa
Application:
Detection/Quantitation, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Anti-Alpha Internexin antibody recognizes alpha-internexin which is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits. Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alpha-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder. The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals. Anti-Alpha Internexin antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Chicken/Avian
Immunogen:
Anti-Alpha Internexin Antibody was produced by repeated immunizations with recombinant rat alpha internexin expressed in E. coli.
Isotype:
IgY
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Dry Ice
Specificity:
Anti-Alpha Internexin Antibody is directed against rat Alpha Internexin proteins. The antibody was purified from chicken eggs as an IgY fraction. This antibody is directed against rat alpha internexin protein. Reactivity is expected from mouse and human. Cross reactivity with Alpha Internexin from other species has not been determined.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunohistochemistry of Anti-Alpha Internexin (chicken) Antibody. Tissue: cultured neurons and glia. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Alpha Internexin antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase chicken secondary antibody at 1:10,000 for 45 min at RT. Localization: Alpha Internexin is neuronal. Staining: Alpha Internexin as precipitated red signal with anti-coronin 1a antibody (green).
Western Blot of Anti-Alpha Internexin (chicken) Antibody. Lane 1: rat cortex lysate. Lane 2: none. Load: 10 ug per lane. Primary antibody: Alpha Internexin antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ chicken secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~66kDa/~66kDa for alpha internexin protein. Other band(s): none.