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Antibodies

Beta-2-Microglobulin Antibody

Product Sizes
100 ug
200-401-141-0100-100UG
About this Product
SKU:
200-401-141-0100
Additional Names:
B2M|rabbit anti-Beta-2-Microglobulin Antibody, b2-Microglobulin, B2M antibody, B2MG_HUMAN antibody, Beta 2 microglobin antibody, Beta 2 microglobulin precursor antibody, Beta chain of mhc class 1 proteins antibody, Hdcma22p antibody, CDABP0092 antibody, IMD43 antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Anti-beta-2-Microglobulin Antibody detects beta-2-Microglobulin. Beta-2-microglobulin is a component of the class I major histocompatibility complex (MHC), which are present on all nucleated cells (excludes red blood cells). It is involved in the presentation of peptide antigens to the immune system. Beta-2-microglobulin associates not only with the alpha chain of MHC class I molecules, but also with class I-like molecules such as CD1 and Qa. Defects in B2M are the cause of hypercatabolic hypoproteinemia. Anti-beta-2-Microglobulin Antibody is ideal for investigators involved in Cell Signaling, Immunology and Cell Biology research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with human beta-2-Microglobulin protein isolated from urine.
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Human
Shipping Conditions:
Ambient
Specificity:
Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum, as well as purifie
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunohistochemistry of Rabbit anti-Beta-2-Microglobulin Antibody. Tissue: normal human skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Left panel: isotype control, Right panel: B Beta2 microglobulin antibody at 1 ug/ml for 20 min at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: B Beta2 microglobulin is cell membrane (and to lesser amount cytoplasmatic compartment). Staining: Beta-2-Microglobulin as brown with diaminobenzidine and with a hematoxylin purple counterstain.
Immunofluorescence Microscopy of Rabbit Anti-Beta-2-microglobulin antibody. Tissue: Human Embryonic Kidney cells (HEK 293 cells). Fixation: 2% paraformaldehyde for 15 minutes. Antigen retrieval: not required. Primary antibody: B Beta-2 microglobulin antibody at 1:200 for overnight at 4°C. Secondary antibody: donkey anti-rabbit secondary antibody conjugated with Alexa Fluor 488 fluorochrome at 1:1,000 for 45 min at RT. Localization: B Beta2-microglobulin is mainly on the cell surface and occasionally cytoplasmic. Staining: Beta-2-microglobulin as green fluorescent signal.
Western Blot of Rabbit Anti-Beta-2-Microglobulin antibody. Lane 1: Beta-2-Microglobulin. Load: 50 ng per lane. Primary antibody: Beta-2-Microglobulin primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 12 kDa, 12 kDa for Beta-2-Microglobulin. Other band(s): Beta-2-Microglobulin splice variants and isoforms.