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Antibodies

Hepatitis Virus A59 Nonstructural Protein 9 Antibody

Product Sizes
100 ug
200-301-A56-100UG
About this Product
SKU:
200-301-A56
Additional Names:
1a|mouse anti-Hepatitis Virus A59 Nonstructural Protein 9 Antibody, mouse anti-MHV-A59 nsp9 protein antibody
Application:
Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
2C6.H1
Clonality:
Monoclonal
Concentration:
2.112 mg/ml
Extra Details:
The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Mouse
Immunogen:
This antibody was produced in mice by repeated immunizations with E.coli derived full-length MHV-A59 nsp9 protein. This protein is part of the viral replicase polyprotein.
Isotype:
IgG2b, IgG2b, kappa
Physical State:
Liquid
Reactivities:
Mouse
Shipping Conditions:
Dry Ice
Specificity:
This antibody is directed against the MHV-A59 nsp9 protein. This product was purified from tissue culture supernatant fluid by Protein A chromatography. No cross reactivity occurs with SARS CoV nsp9. Cross reactivity with homologues from other sources has not been tested.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Monoclonal Antibody
C. Immunoblot analysis of (p/n 200-301-A56, 1:1000) non-structural protein 9 (NSP9) precursor (P50 and P37) and mature forms (P13) in iBMDMs following the indicated treatment. Blots were reprobed for GAPDH to serve as the internal control. Fig 4. PMID: 37864059.
Immunofluorescence microscopy using Rockland Immunochemical's anti-MHV-A59 nsp9 antibody, 6-h post infection in mouse L cells. Cells were fixed in 3% para-formaldehyde. For detection Cy2 conjugated Goat-anti-Mouse IgG MX10 (610-111-121) was used. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.
Western blotting using Rockland's anti-MHV-A59 nsp9 antibody to detect protein in various lysates, 6h post MHV infection. Lane 1 shows no cross-reactivity with SARS-CoV-infected Vero cells. Negative controls (lanes 2 and 4) show no staining. Specific reactivity against MHV-A59 nsp9 from infected mouse L cells is shown in lane 3. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.