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Antibodies

Chicken Red Blood Cell RBC Antibody

Product Sizes
2 ml
103-4139-2ML
About this Product
SKU:
103-4139
Additional Names:
Anti-RBC antibody, Red Blood Cell Antibody, Antibody for hemagglutination, rabbit anti RBC, rabbit anti-chicken Red Blood Cells (RBC), haemolysin, hemolysin, erythrocytes sensitizing agent
Application:
Agglutination, Bioactivity, Detection/Quantitation, Immunofluorescence, Immunohistochemistry
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
60 mg/ml
Extra Details:
Anti-CHICKEN Red Blood Cell Antibody may be used in hemagglutination assays. Haemagglutination assay or HA is a method of quantification for viruses or bacteria by hemagglutination. Some viral families and many bacteria have envelope or surface proteins which are able to agglutinate (stick to) human or animal red blood cells (RBC) and bind to N-acetylneuraminic acid. As each of the agglutinating molecule attaches to multiple RBCs, a lattice-structure will form. Normally, a virus dilution (e.g. 2-fold from 1:4 to 1:4096) will be applied to an RBC dilution (e.g. 0.1% to 0.7% in steps of 0.2%) for approx. 30 min, often at 4° C, otherwise viruses with neuraminidase activity will detach the virus from the RBCs. Then the lattice forming parts will be counted and the titer calculated. The titer of a hemagglutination assay is determined by the last viable "lattice" structure found. This is because it is at the point where, if diluted anymore, the amount of Virus particles will be less than that of the RBCs and thus not be able to agglutinate them together. Anti-CHICKEN Red Blood Cell Antibody is used to sensitize erythrocytes and quantitate agglutination. PLEASE READ: This product contains blood so may require extra documentation and/or clearances, please contact sales@2bscientific.com for further information and a more Accurate quote and lead time
Format:
Antiserum
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Chicken washed pooled Red Blood Cells (RBC)
Physical State:
Lyophilized
Shipping Conditions:
Ambient
Specificity:
This product was prepared from polyspecific antiserum by a delipidation and defibrination.
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Therapeutic efficacy of anti-human P mAb 19.1 in a murine model of extravascular hemolysisa: Pharmacodynamics of mAb 19.1 in hP transgenic mice. Each mouse was treated with 0.5 mg mAb 19.1 (n=3 mice). Serum samples were collected before and at various time points after mAb treatment and assessed for LPS-dependent AP complement activity. At this dosage of mAb 19.1, AP complement activity was suppressed to background (P-/-) level for 2 days. EDTA: time 0 sample with EDTA (20 mM) added. NS, non-significant comparing 8, 24 and 48 hr samples with fP-/- or EDTA-treated serum, or comparing 72 hr sample with 0 hr sample. One-way ANOVA. b: Pharmacodynamics of anti-mouse C5 mAb (BB5.1) in hP transgenic mice. Each mouse was treated with 1mg of anti-mouse C5 mAb (n=3). Serum samples were collected before and at various time points after mAb treatment and assessed for lytic activity using antibody-sensitized chicken RBCs. C5 knockout (KO) mouse serum was used as a control for C5 inhibition. Percentage of chicken RBC (cRBC) lysis was normalized to a sample completely lysed by hypotonic shock in double distilled water. * p<0.0001, NS: non-significant compared with C5KO serum. One-way ANOVA. c. Effect of anti-hP mAb 19.1 on the survival of transfused CFSC-labeled DAF-/-/Crry-/-/C3-/- mouse erythrocytes in hP- transgenic mice. Recipient mice were treated with anti-hP mAb 19.1 (n=4) or an isotype control mAb (n=4) or anti-mouse C5 mAb (n=3) 6 hours prior to red blood cell transfusion and blood samples were taken at 5 min, 6 hrs and then daily for 5 days. The percentage of CFSC-labeled red blood cells was measured by FACS and normalized to that determined at 5 min (100%). Transfused DAF-/-/Crry-/-/C3-/- mouse red blood cells were rapidly eliminated in control mAb- or anti-C5 mAb-treated hP transgenic mice but such an outcome was prevented by mAb 19.1 treatment. * p< 0.001, Two-way ANOVA. d. FACS analysis of activated C3 fragment deposition on DAF-/-/Crry-/-/C3-/- RBCs 5 and 30 min after their transfusion into control mAb- or mAb 19.1-treated hP-Tg/P-/- mice (representative data from two recipient mice are shown). At both time points, C3 fragment deposition was significantly higher on RBCs transfused into control mAb-treated than mAb 19.1-treated hP-Tg/P-/- mice. The reason for the marked reduction in C3 fragment deposition on RBCs in control mAb-treated hP-Tg/P-/- mice between 5 and 30 min is unknown, but could be caused by C3 fragment degradation to C3d or shedding from the cell surface or rapid removal of the C3-opsonized cells. Data in a-c are presented as mean (SD) with indicated n numbers. Antibody-sensitized chicken RBC (p/n R401-0050) prepared by incubating the cells with a rabbit anti-chicken RBC antibody (p/n 103-4139). Fig 4. PMID: 29898960.
Confocal images of infected chicken blood cells stained with anti-red blood cell antibodies. Anti-RBC, images stained with rabbit anti-chicken red blood cell antibody 103-4139; DIC, differential interference contrast images; Hoechst, Hoechst dye staining of nuclei (DNA); Merged, merged images of anti-RBC, DIC, and DAPI. (A-H) Two cells infected with L. sabrazesi gametocytes have some red dots (yellow arrowheads) that appear to be within the cytoplasm of the host cells. (E-H) A strongly stained white cell (grey arrowheads) that has two nuclei and rough granules in the cytoplasm, suggesting heterophils, monocytes, macrophages, or eosinophils. (I-L) A small cell that has a round nucleus and red dots similar (green arrowheads) to those seen in the infected cells. The small size of the cell suggests that it is likely a thrombocyte. The red ruler in each image indicates 10 um. Figure provided by CiteAb. Source: PLoS One, PMID: 26218846.
Confocal images of infected chicken blood cells stained with anti-red blood cell antibodies.Anti-RBC, images stained with rabbit anti-chicken red blood cell antibody 103-4139; DIC, differential interference contrast images; Hoechst, Hoechst dye staining of nuclei (DNA); Merged, merged images of anti-RBC, DIC, and DAPI. (A-H) Two cells infected with L. sabrazesi gametocytes have some red dots (yellow arrowheads) that appear to be within the cytoplasm of the host cells. (E-H) A strongly stained white cell (grey arrowheads) that has two nuclei and rough granules in the cytoplasm, suggesting heterophils, monocytes, macrophages, or eosinophils. (I-L) A small cell that has a round nucleus and red dots similar (green arrowheads) to those seen in the infected cells. The small size of the cell suggests that it is likely a thrombocyte. The red ruler in each image indicates 10 um. Figure provided by CiteAb. Source: PLoS One, PMID: 26218846.
Confocal images of infected chicken blood cells stained with anti-red blood cell antibodies.Anti-RBC, images stained with rabbit anti-chicken red blood cell antibody 103-4139; DIC, differential interference contrast images; Hoechst, Hoechst dye staining of nuclei (DNA); Merged, merged images of anti-RBC, DIC, and DAPI. (A-H) Two cells infected with L. sabrazesi gametocytes have some red dots (yellow arrowheads) that appear to be within the cytoplasm of the host cells. (E-H) A strongly stained white cell (grey arrowheads) that has two nuclei and rough granules in the cytoplasm, suggesting heterophils, monocytes, macrophages, or eosinophils. (I-L) A small cell that has a round nucleus and red dots similar (green arrowheads) to those seen in the infected cells. The small size of the cell suggests that it is likely a thrombocyte. The red ruler in each image indicates 10 um. Figure provided by CiteAb. Source: PLoS One, PMID: 26218846.