14-3-3 sigma protein-GST fusion
Product Sizes
20 ug
009-001-R31S-20UG
About this Product
- SKU:
- 009-001-R31S
- Additional Names:
- 14-3-3 sigma, SFN, stratifin, YWHAS|YWHAS
- Application:
- Western Blot
- Buffer:
- See application note.
- CE/IVD:
- RUO
- Concentration:
- 0.2 ug/ul
- Extra Details:
- 14-3-3S Sigma or stratifin is a protein that is strongly induced by gamma irradiation and other DNA-damaging agents (1). The induction of 14-3-3S Sigma is mediated by a p53 responsive element. Exogenous introduction of 14-3-3S Sigma into cycling cells results in a G2 cell cycle arrest (2). Knockout of 14-3-3S Sigma in cells showed that the cells are unable to maintain cell cycle arrest after DNA damage. The 14-3-3S Sigma -/- cells die as they enter mitosis. This process is associated with a failure of the 14-3-3S Sigma -deficient cells to sequester the proteins that initiate mitosis and prevent them from entering the nucleus. Thus, 14-3-3S Sigma plays an important role in maintaining the G2 checkpoint in cells and preventing mitotic death. 14-3-3S Sigma Protein is ideal for investigators involved in Cell Stress& Chaperone Proteins, AKT/PKB Pathway, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Neurobiology, PKA/PKC Pathway, and WNT Signaling research.
- Immunogen:
- YWHAS
- Physical State:
- Liquid
- Shipping Conditions:
- Dry Ice
- Specificity:
- Recombinant full-length human 14-3-3S Sigma was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
- Storage Conditions:
- -70[o]C Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Rockland Inc
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=009-001-R31S