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Antibodies

Bovine IgG Fc

Product Sizes
1 mg
001-0103-1MG
About this Product
SKU:
001-0103
Additional Names:
Bovine IgG F(c) Agarose Conjugate, Bovine IgG Fc Agarose, Bovine Fc fragment Agarose conjugate
Application:
Dot Blot, SDS-PAGE
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Concentration:
1 mg/ml
Extra Details:
Bovine IgG F(c) Fragment Agarose Conjugated product is made from IgG, secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. This product possesses the F(c) region, recognized by high-affinity Fc receptor proteins followed by agarose conjugation.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype:
IgG
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Serum, anti-Bovine IgG and anti-Bovine IgG F(c). No
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
SDS-PAGE for Bovine IgG F(c) Fragment. Lane 1: 1ug Reduced Bovine Fc. Lane 2: MW ladder Opal pre-stained (p/n MB-210-0500). Lane 3: 1ug Non-Reduced Bovine Fc. Coomassie Blue Stained.
Dot Blot of Bovine IgG F(c) Fragment. Lane 1: 100 ng Bovine Fc. Lanes 2-5: serial dilution 3 fold of Antigen. Primary Antibody: Rabbit Anti-Bovine Fc (p/n 601-4103) at 1 ug/ml at RT for 1 hour. Secondary Antibody: Rabbit Secondary HRP Antibody (p/n 611-103-122) at 1:70,000 for 30 min at RT. Block: MB-070 at RT for 30 minutes.