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Molecular Biology

pY-SUMOstar Pichia Secretory Vector

Product Sizes
pY-SUMOstar Pichia Secretory Vector
£652.00
PY-2161-0020
Academic Pricing
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About this Product
SKU:
PY-2161-0020
Extra Details:
The pY-secSUMOstar vector enables expression and secretion of SUMOstar fusions in Pichia pastoris. Protein expression is driven by the primary alcohol oxidase (AOX1) promoter, which is both tightly regulated and highly inducible. The alpha mating factor (S. cerevisiae) targets the fusion protein to the secretory pathway, while both a FLAG and a His6 tag preceed SUMOstar for convenient purification. Chromosomal integration is accomplished by DNA linearization within the promoter using a variety of convient endonuclease restriction sites, while mult-copy integrants can be obtained through strain selection against increasing concentrations of the antibiotic Zeocin.
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Size:
20 ug
Supplier:
LifeSensors
Type:
Molecular Biology Reagents: Vector