Atto647N-LPETGG
Product Sizes
Atto647N-LPETGG
£176.00
FP-030
About this Product
- SKU:
- FP-030
- Extra Details:
- Atto647N-LPETGG is the sortase recognition sequence, LPETGG, coupled to the fluorescent label Atto647N. Atto647N-LPETGG can be used for site-specific labeling of target proteins using sortase-catalyzed transpeptidation. In this process, sortase A cleaves between the thr and gly residues, forming a thioester intermediate with the peptide probe. A protein N-terminally engineered with a series of glycine residues resolves this intermediate, regenerating the active site cysteine of sortase and conjugating the probe to the N-terminus of the protein. Atto647N-LPETGG contains the Atto647N fluorescent label which is efficiently excited in the range 625 - 660 nm, with strong absorption, high fluorescence quantum yield and high photo-stability.
- Molecular Weight:
- 1200.6
- Purity:
- >95% by HPLC
- References:
- <p>Tanaka et al (2008) Site-specific protein modification on living cells catalyzed by Sortase. Chembiochem.<strong> 9</strong>(5) 802 PMID: 18297670 </p><p>Popp and Ploegh (2011) Making and Breaking Peptide Bonds: Protein Engineering Using Sortase. Angew. Chem. Int. Ed.<strong> 50</strong> 5024 PMID: 21538739 </p><p>Theile et al (2013) Site-specific N-terminal labeling of proteins using sortase-mediated reactions. Nat. Protoc. <strong>8</strong>(9) 1800 PMID: 23989674</p>
- Sequence:
- Atto647N-Leu-Pro-Glu-Thr-Gly-Gly-OH
- Shipping Conditions:
- Ambient
- Storage Conditions:
- Store dry, frozen and in the dark
- Size:
- 0.1 mg
- Supplier:
- Isca Biochemicals
- Solubility:
- Soluble in water
- Formula:
- C65H85N9O13