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Antibodies

Anti-PD-L1 (Extracellular region) M051 Antibody

Product Sizes
100 ul
PM0511-100UL
About this Product
SKU:
PM0511
Additional Names:
Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1, B7 homolog 1, B7-H1, CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1
Application:
Immunocytochemistry, Immunoprecipitation, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
M051
Clonality:
Monoclonal
Extra Details:
Our PD-L1 (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human PD-L1 (Extracellular region) and is Protein G purified. It is great for use in WB, ICC, IP.
Format:
Protein G Purified
Gene Details:
CD274
Host:
Mouse
Immunogen:
Clone (M051) was generated from a recombinant protein that included the extracellular region of human PD-L1 protein.
Isotype:
IgG1
Molecular Weight:
45-55
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Blue Ice
Specificity:
Clone M051 mouse monoclonal antibody detects a 45-55 kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human recombinant PD-L1 extracellular region, and human lung tissue. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Monoclonal Antibody
Representative Standard Curve using mouse monoclonal anti-PD-L1 (PM0511) for ELISA capture of human recombinant PD-L1 extracellular region with a His-tag. Captured protein was detected by suitable anti-His-tag antibody followed by appropriate secondary antibody HRP conjugate.
Immunocytochemical labeling of PD-L1 in aldehyde fixed human MDA-MB-231 carcinoma cells untreated (A) or treated with 100 ng/ml IFNG Gamma for 48 hrs (B). The cells were labeled with mouse monoclonal anti-PD-L1 (PM0511). The antibody was detected using goat anti-mouse DyLight® 594.
Western blot of human recombinant PD-L1 extracellular region (40 kDa; lanes 1 & 3) and endogenous PD-L1 in human lung (lanes 2 & 4). The blot was probed with mouse monoclonals anti-PD-L1 (PM0511) at 1:500 (lanes 1 & 2) and anti-PD-L1 (PM0801) at 1:250 (lanes 3 & 4).