Raf1 RBD Protein, Human, Recombinant, Biotinylated
Product Sizes
0.1 mg
RAF1-B-301-0.1MG
About this Product
- SKU:
- RAF1-B-301
- Application:
- Protein Engineering
- Conjugate:
- Biotin
- Extra Details:
- Ras Binding Domain (RBD) of Human Raf-1 Proto-Oncogene, Serine/Threonine Kinase (RAF1), (UniProt # P04049, amino acids 50-140) with the N-terminal AviTag™ peptide and His-tag is produced in E. coli cells and site-specifically biotinylated at AviTag with BirA enzyme (https://amidbiosciences.com/collections/proteins/products/bira-biotin-ligase). The Raf1 RBD protein binds specifically to GTP-bound Ras proteins. Ras proteins, like other small GTPases, regulate molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Ras binds specifically to the Ras-binding domain (RBD) of Raf1 to control downstream signaling cascades. The affinity between RBD and GDP-bound Ras is three orders of magnitude lower (1). Therefore, the RBD protein can be used to specifically precipitate active, GTP-bound Ras as well as to specifically block the activity of Ras in vitro and in vivo. Applications Measurement of the GTP/GDP bound ratio of Ras in vitro. Quantitation of GTP-bound Ras from tissue and tissue culture cell lysates. RAS/RAF binding studies. Inhibitor screening for RAS/RAF interaction MW: ~13.4 kDa Tag(s): The N-terminal 6XHis and AviTag™ Amino Acids: 50-140 of Raf1, UniProt # P04049
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -70[o]C
- Supplier:
- Amid Biosciences
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
- Manufacturer's Data Sheet:raf1-rbd-50-140-protein-human-recombinant-biotinylated
