Phage Display ER2738 Chemically Competent E. coli Cells
Product Sizes
10 x 0.1 ml
ER-201-10X0.1ML
About this Product
- SKU:
- ER-201
- Extra Details:
- The E. coli strain ER2738 is an amber suppressor (glnV) F+ strain with a rapid growth rate and is particularly well-suited for M13 phage propagation. Amid Biosciences' high efficiency chemically competent ER2738 cells can be used for phage display, antibody phage display library construction and screening. The F-factor of ER2738 contains a mini-transposon which confers tetracycline resistance, so cells can be selected by plating and propagating in tetracycline-containing medium. ER2738 cultures for infection can be grown either in LB or LB+Tet media. Tetracycline does not need to be added to media during phage amplification. Loss of F-factor in nonselective media is insignificant as long as cultures are not serially diluted. Although ER2738 is a recA+ strain, there are no reports on spontaneous in vivo recombination events with M13 or phagemid vectors. ER2738 cells are recommended for use with New England Biolab's Ph.D.™ Phage Display Kits (1). ER2738 chemically competent cells are supplied as a pack of 10 convenient 100 ul per tube aliquots (sufficient for 20 reactions). ER2738 electrocompetent E.coli cells are also available. Bulk quantities and custom packaging are available at very competitive prices for all Competent Cells. Catalog # ER-201 Genotype: F'proA+B+ lacIq D Delta(lacZ)M15 zzf::Tn10(TetR)/ fhuA2 glnV D Delta(lac-proAB) thi-1 D Delta(hsdS-mcrB)5 Features and Benefits Transformation efficiency > 109 transformants /ug pBR322 Useful for preparation of phage display libraries Fast-growing Amber suppressor (supE): UAG>CAG(Gln) Storage: at -80°C.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -70[o]C
- Supplier:
- Amid Biosciences
- Type:
- Cells: Non-Human Cells
