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5-alpha Electrocompetent E. coli Cells

Product Sizes
10 X 0.05 ml/tube
EAB5-201-10X0.05ML/TUBE
10 x 0.05 ml
EAB5-201-10X0.05ML
About this Product
SKU:
EAB5-201
Application:
Protein Engineering
Extra Details:
High efficiency electrocompetent AB 5-alpha E.coli cells are comparable to the DH5A Alpha™ strain of E. coli. AB 5-alpha cells carry recA1 and endA1 mutations that minimize recombination and improve plasmid stability and quality. They also contain a lacZ marker that provides A Alpha-complementation of the B Beta-galactosidase gene for blue/white color screening and are ideal for cloning, sub-cloning, library construction, and plasmid isolation. Transformation efficiency ≥ 1x1010 cfu/ug pUC19 plasmid DNA. E. coli genotype: F- endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) deoR supE44 phoA D Delta(lacZYA-argF) U169 F Phi80lacZD DeltaM15 L Lambda- thi-1 AB 5-alpha electrocompetent cells are supplied as a pack of 10 single-use 50 ul/tube aliquots. Catalog # EAB5-201 Quality Control Testing: Transformations are performed using 50 ul aliquots of cells and 50 pg of pUC19 control plasmid. Storage: Transfer the cells directly from the dry ice shipping container to the -80°C freezer. AB 5-alpha Chemically Competent E.coli Cells are also available.
Shipping Conditions:
Dry Ice
Storage Conditions:
-70[o]C
Supplier:
Amid Biosciences
Type:
Cells: Non-Human Cells