oYo-Link[R] Azide-TAMRA Antibody Labeling Reagent
Product Sizes
100 ug
AT3002-TAMRA-100UG
About this Product
- SKU:
- AT3002-TAMRA
- Application:
- Conjugation/Labeling
- Buffer:
- PBS
- Conjugate:
- TAMRA
- Concentration:
- 270 ug/ml
- Extra Details:
- NOTE: It is important to perform the click-reaction with DBCO-x first, prior to antibody conjugation. UV illumination will damage the Azide activity and thus antibody conjugation should only be performed after the click-reaction. Research Use Only Applications: Click Chemistry; Fluorescent imaging and/or quantification of oYo-Link[R] following conjugation or surface immobilization (e.g. to nanoparticles) oYo-Link[R] Azide allows for the site-specific labeling of oYo-Link[R]-compatible antibodies with up to two azide labels per antibody for subsequent click chemistry reactions. oYo-Link[R] Azide limits the attachment of the azide labels to the heavy chains of the antibody and therefore ensures that the azide label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 azide labels to be attached per antibody, providing precise antibody-conjugate ratios.The antibody labeling procedure simply requires mixing oYo-Link[R] Azide with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table) . Requirements: To label your compatible antibodies with oYo-Link[R], you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com
- Molecular Weight:
- 8 kDa
- Physical State:
- Dried pellet
- Shipping Conditions:
- Ambient
- Storage Conditions:
- 2-8[o]C
- Supplier:
- AlphaThera
- Type:
- Buffers & General Consumables: Others