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Biotinylated SARS-CoV-2 Spike Trimer Protein (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R, D950N), His,Avitag[TM] (MALS verified)

Product Sizes
25 ug
SPN-C82EC-25UG
200 ug
SPN-C82EC-200UG
About this Product
SKU:
SPN-C82EC
Additional Names:
S glycoprotein|S protein|Spike|Spike glycoprotein
Buffer:
PBS
Conjugate:
Biotin
Extra Details:
Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag (SPN-C82Ec) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The mutations (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R, D950N) were identified in the SARS-CoV-2 Delta variant (Pango lineage: B.1.617.2; other names: 21A/S:478K). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
Immunogen:
Spike protein
Molecular Weight:
139.5 kDa
Physical State:
Powder
Shipping Conditions:
Ambient
Storage Conditions:
-20[o]C
Supplier:
ACRO Biosystems
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Conjugated Protein
Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag (Cat. No. SPN-C82Ec) at 1 ug/mL (100 uL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 ug/well) plate can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-2 ng/mL (QC tested).
Immobilized Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag (Cat. No. SPN-C82Ec) at 1 ug/mL (100 uL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 ug/well) plate can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122) with a linear range of 0.1-1 ng/mL (Routinely tested).