Anti-Ms CD4 FITC
Product Sizes
0.1 mg
AFC-1772VY-0.1MG
About this Product
- SKU:
- AFC-1772VY
- Additional Names:
- T4/Leu-3, L3T4
- Application:
- Flow Cytometry
- Buffer:
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- translate.label.attr.clone:
- GK1.5
- Clonality:
- monoclonal
- Conjugate:
- FITC
- Concentration:
- 0.5 mg/ml
- Extra Details:
- CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
- Format:
- FITC
- Immunogen:
- Mouse CTL clone V4 cells
- Isotype:
- IgG2b
- Purification:
- Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Research Area:
- Immunology
- Shipping Conditions:
- Blue Ice
- Specificity:
- The rat monoclonal antibody GK1.5 reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
- Storage Conditions:
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
- Supplier:
- Nordic BioSite
- Manufacturer's Data Sheet:Anti-Ms CD4 FITC