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Antibodies

Anti-MeCP2 (C-terminus) Antibody

Product Sizes
100 ul
MP4591-100UL
About this Product
SKU:
MP4591
Additional Names:
Methyl-CpG-Binding2
Application:
Immunocytochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Our MeCP2 (C-terminus) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat MeCP2 (C-terminus) and is antigen affinity purified. It is great for use in WB, ICC.
Format:
Antigen Affinity Purified
Gene Details:
MECP2
Host:
Rabbit
Immunogen:
MeCP2 synthetic peptide (coupled to KLH) corresponding to amino acid residues at the C-terminus of mouse MeCP2. This peptide sequence is highly conserved in rat and human MeCP2, and has low homology to other nuclear proteins.
Isotype:
IgG
Molecular Weight:
75
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects a 75 kDa* protein corresponding to the molecular mass of MeCP2 on SDS-PAGE immunoblots of human PC3 cells, rat PC12 cells, and mouse brain tissue.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Immunocytochemical labeling of MeCP2 in rat PC12 cells differentiated with NGF. The cells were probed with MeCP2 (C-terminus) rabbit polyclonal antibody (MP4591) in the absence (left) or presence (right) of blocking peptide (MX4595). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
Western blot of adult mouse brain tissue lysate. The blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonals anti-MeCP2 (Ser-80) (lanes 1 & 2) or anti-MeCP2 (C-terminus) (lanes 3 & 4).