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Antibodies

Anti-CREB (Ser-271), Phosphospecific Antibody

Product Sizes
100 ul
CP4161-100UL
About this Product
SKU:
CP4161
Additional Names:
Cyclic AMP, CREB
Application:
Immunocytochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Ser-271 - Phosphorylated
Extra Details:
Our CREB (Ser-271) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat CREB (Ser-271) and is antigen affinity purified. It is great for use in WB, ICC.
Format:
Antigen Affinity Purified
Gene Details:
CREB1
Host:
Rabbit
Immunogen:
Phospho-CREB (Ser-271) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-271 in human CREB. This sequence is well conserved in rat, mouse, chicken, and zebrafish CREB. The site has some homology to the conserved site in ATF1 (Ser-198) and CREM (Ser-287).
Isotype:
IgG
Molecular Weight:
43
Physical State:
Liquid
Reactivities:
Avian, Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects a 43 kDa* protein corresponding to the molecular mass of CREB on SDS-PAGE immunoblots of human A431, Jurkat, and rat PC12 cells treated with Calyculin A, but is not observed after lambda phosphatase treatment.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Western blot analysis of human A431 cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-CREB (N-terminal region) (lanes 1 & 2), anti-CREB (Ser-133) (lanes 3 & 4), and anti-CREB (Ser-271) (lanes 5 & 6).
Immunocytochemical labeling of phosphorylated CREB in control and calyculin A-treated A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40 before labeling with rabbit polyclonal CREB (Ser-271). The antibody was detected using goat anti-rabbit DyLight® 594.