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Antibodies

Anti-CREB (N-terminal region) Antibody

Product Sizes
100 ul
CP4141-100UL
About this Product
SKU:
CP4141
Additional Names:
Cyclic AMP, CREB
Application:
Immunocytochemistry, Immunoprecipitation, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Our CREB (N-terminal region) primary antibody from PhosphoSolutions is rabbit polyclonal. It detects chicken, human, mouse, and rat CREB (N-terminal region) and is antigen affinity purified. It is great for use in WB, ICC, IP.
Format:
Antigen Affinity Purified
Gene Details:
CREB1
Host:
Rabbit
Immunogen:
CREB synthetic peptide (coupled to carrier protein) corresponding to amino acids in the N-terminal region of human CREB. This sequence is well conserved in rat, mouse, and chicken CREB, and has low homology to other CREB family members.
Isotype:
IgG
Molecular Weight:
43
Physical State:
Liquid
Reactivities:
Avian, Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects a 43 kDa* protein corresponding to the molecular mass of CREB on SDS-PAGE immunoblots of human A431, Jurkat, HeLa, and rat PC12 cells.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Immunocytochemical labeling of CREB in A431 that were fixed in paraformaldehyde and permeabilized using NP-40. The cells were labeled with rabbit polyclonal CREB (N-terminal region). The antibody was detected using goat anti-rabbit DyLight® 594.
Western blot analysis of human A431 cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3 & 5) then the blots were treated with lambda phosphatase (lanes 2, 4 & 6). The blots were probed with anti-CREB (N-terminal region) (lanes 1 & 2), anti-CREB (Ser-133) (lanes 3 & 4), and anti-CREB (Ser-271) (lanes 5 & 6).