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Antibodies

Anti-CREB (Ser-133), Phosphospecific Antibody

Product Sizes
100 ul
CM4151-100UL
About this Product
SKU:
CM4151
Additional Names:
Cyclic AMP, CREB
Application:
Immunocytochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
M415
Clonality:
Monoclonal
Conjugate:
Ser-133 - Phosphorylated
Extra Details:
Our CREB (Ser-133) phosphospecific primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, and rat CREB (Ser-133) and is Protein A purified. It is great for use in WB, ICC.
Format:
Protein A Purified
Gene Details:
CREB1
Host:
Mouse
Immunogen:
Clone M415 was generated from phospho-CREB (Ser-133) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-133 in human CREB. This sequence is well conserved in rat, mouse, chicken, and zebrafish CREB. The site is also conserved in CREM (Ser-136) and ATF1 (Ser-63).
Isotype:
IgG1
Molecular Weight:
43
Physical State:
Liquid
Reactivities:
Avian, Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects a 43 kDa* protein corresponding to the molecular mass of CREB on SDS-PAGE immunoblots of human A431, Jurkat, and rat PC12 cells treated with Calyculin A, as well as A431 treated with EGF and PC12 treated with NGF or Forskolin. This reactivity is not observed after lambda phosphatase treatment.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Monoclonal Antibody
Immunocytochemical labeling of phosphorylated CREB in calyculin A-treated A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40 before labeling with mouse monoclonal CREB (Ser-133). The antibody was detected using goat anti-mouse DyLight® 594.
Western blot analysis of human A431 cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3 & 5) then the blots were treated with lambda phosphatase (lanes 2, 4 & 6). The blots were probed with anti-CREB (N-terminal region) (lanes 1 & 2), anti-CREB (Ser-133) (lanes 3 & 4), and anti-CREB (Ser-271) (lanes 5 & 6).