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Antibodies

Atm Recombinant Antibody

Product Sizes
50 ul
CAC10058-50UL
100 ul
CAC10058-100UL
About this Product
SKU:
CAC10058
Additional Names:
A-T mutated|Ataxia telangiectasia mutated|ATM|Serine-protein kinase ATM
Application:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
phosphate buffered saline
CE/IVD:
RUO
translate.label.attr.clone:
Monoclonal, Recombinant Monoclonal
Clonality:
Recombinant Monoclonal
Extra Details:
The Atm Recombinant Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IP.
Immunogen:
ATM
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Antibody: Recombinant Antibodies
Western Blot; Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate; All lanes: ATM antibody at 2.05ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 350 KDa; Observed band size: 350 KDa
IHC image diluted at 1:205 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:205 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating ATM in PC3 whole cell lysate; , Lane 1: Rabbit control IgG instead in PC3 whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC10058 (3ug) + PC3 whole cell lysate (500ug); , Lane 3: PC3 whole cell lysate (20ug)