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Antibodies

Cd21 Recombinant Antibody

Product Sizes
50 ul
CAC10016-50UL
100 ul
CAC10016-100UL
About this Product
SKU:
CAC10016
Additional Names:
C3DR|CD21|Complement C3d receptor|Complement receptor type 2|Cr2|EBV receptor|Epstein-Barr virus receptor
Application:
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
phosphate buffered saline
CE/IVD:
RUO
translate.label.attr.clone:
16F10
Clonality:
Recombinant Monoclonal
Extra Details:
The Cd21 Recombinant Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF, FC.
Immunogen:
CR2
Isotype:
IgG
Physical State:
Liquid
Purification:
Affinity Purified
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
Biomatik
Type:
Antibody: Recombinant Antibodies
Western Blot; Positive WB detected in Raji whole cell lysate; All lanes CD21 antibody at 0.55ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 155 KDa; Observed band size: 155 KDa
IHC image of CY5710 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Raji cells with CAC10016 at 1:34,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).