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Antibodies

anti-ATP5G1 + ATP5G2 + ATP5G3 antibody

Product Sizes
50 ug
ARG42697-50UG
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
ARG42697
Additional Names:
ATP5G1: ATP5A; ATP5G; ATP synthase F(0) complex subunit C1, mitochondrial; ATP synthase lipid-binding protein; ATP synthase proteolipid P1; ATP synthase proton-transporting mitochondrial F(0) complex subunit C1; ATPase protein 9; ATPase subunit cATP5G2: ATP5A; ATP synthase F(0) complex subunit C2, mitochondrial; ATP synthase lipid-binding protein; ATP synthase proteolipid P2; ATP synthase proton-transporting mitochondrial F(0) complex subunit C2; ATPase protein 9; ATPase subunit cATP5G3: P3; ATP synthase F(0) complex subunit C3, mitochondrial; ATP synthase lipid-binding protein; ATP synthase proteolipid P3; ATP synthase proton-transporting mitochondrial F(0) complex subunit C3; ATPase protein 9; ATPase subunit c
Application:
Flow Cytometry, IHC-Paraffin, Western Blot
Buffer:
0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
0.5 mg/ml
Extra Details:
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]
Host:
Rabbit
Immunogen:
Recombinant protein corresponding to D62-L113 of Human ATP5G1.
Isotype:
IgG
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Polyclonal Antibody
Flow Cytometry: HL-60 cells were blocked with 10% normal goat serum and then stained with ARG42697 anti-ATP5G1 + ATP5G2 + ATP5G3 antibody (blue) at 1 ug/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 ug/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
Flow Cytometry: PC-3 cells were blocked with 10% normal goat serum and then stained with ARG42697 anti-ATP5G1 + ATP5G2 + ATP5G3 antibody (blue) at 1 ug/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 ug/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
Immunohistochemistry: Paraffin-embedded Human intestinal tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42697 anti-ATP5G1 + ATP5G2 + ATP5G3 antibody at 2 ug/ml dilution, overnight at 4°C.
Immunohistochemistry: Paraffin-embedded Human ovarian cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42697 anti-ATP5G1 + ATP5G2 + ATP5G3 antibody at 1 ug/ml dilution, overnight at 4°C.
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42697 anti-ATP5G1 + ATP5G2 + ATP5G3 antibody at 1 ug/ml dilution, overnight at 4°C.