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Founded in 1981 with the vision of creating superior veterinary diagnostics kits and related reagents, VMRD boasts the most comprehensive portfolio of veterinary diagnostics products worldwide. These include USDA-licensed ELISA kits, immunofluorescence reagents, antibodies, and Coombs testing reagents, with a strong focus on canine, feline, bovine, porcine, and equine. VMRD has a strong market reputation for delivering best in class products and services and, as a result, has had a global impact on improvements in animal welfare. At 2BScientific, we frequently receive enquiries from researchers seeking veterinary diagnostic products to monitor disease, and we have no hesitation in endorsing VMRD to supply these. Recently, we’ve recommended VMRD’s ELISA kits to laboratories wishing to accurately detect antibodies specific to conditions including bluetongue and foot and mouth disease, and we’ve also suggested VMRD’s antibodies to monitor antigens such as canine distemper and feline immunodeficiency virus.

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  • 100% guaranteed
  • ISO Certified

A new serology solution from VMRD for Johne’s disease

Johne’s disease is a chronic and highly contagious wasting disease of all ruminants caused by Mycobacterium avium ssp. paratuberculosis (MAP). Major clinical signs include weight loss, pipestream diarrhea, edema, decreased milk production, and eventually death.

In the US, Johne’s disease affects ~70% of dairy herds[1], costing the industry up to $250 million per year in production loss[2]. An estimated 5-10% of beef herds are also affected[3]. Currently, no effective vaccines or specific treatments are available, making proper diagnostic testing pivotal for management efforts.
[1] USDA. NAHMS - Dairy, 2008. [2] Ott SL, et al. Prev Vet Med, June 1999. [3] USDA. NAHMS - Beef, 1999.

Performance for validated samples
Bovine serum*

* USDA licensed  
433 positive samples VMRD: 93.1% Se, 90% Sp
261 negative samples Competitor A: 91.0% Se, 85.4% Sp
(confirmed by fecal culture & PCR) Competitor B: 86.6% Se, 89.3% Sp

User-friendly qualities

  • No pre-incubation step for absorption
  • Results in <75 min
  • Ready-to-use conjugate and controls
  • Straightforward reagent preparation
  • Simple reagent identification by color
  • Reliable reagent consistency – no precipitation

Research data
Bovine milk

  Sensitivity Specificity
VMRD 84.7% 82.1%
Competitor A 73.6% 79.9%
Based on 59 positive and 224 negative samples with status confirmed by faecal culture and PCR

Research data
Caprine serum

  Sensitivity Specificity
VMRD 68.6% 100%
Competitor B 62.9% 100%
Competitor C 52.9% 100%
Based on 70 positive and 10 negative samples with status confirmed by necropsy
  Sensitivity Specificity
VMRD 100% 99.2%
Competitor A 92.6% 99.2%
Competitor B 100% 98.5%
Based on 27 positive and 129 negative samples with status confirmed by culture and/or PCR

Taking control of Johne’s Disease
with ELISA testing

  • Cost-effective for assessing herd prevalence and risk
  • Identifies target areas for focused management efforts
  • Highly sensitive nd specific for diagnosis of clinically affected animals

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