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BCIP/NBT Substrate Kit, Alkaline Phosphatase (AP), (5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium)

Product Size(s)
1 Kit
Academic Pricing
Please contact us for academic pricing.
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About this Product
Additional Names:
Enzyme Substrates [Enzyme Substrates ], Precipitating AP substrate, Fluorecent enzyme substrate, Colourimetric enzyme substrate
Immunohistochemistry, IHC-Frozen, IHC-Paraffin, Immunocytochemistry, ELISPOT, FLUOROSPOT
Extra Details:


  • Greater sensitivity than conventional substrates
  • Consistent and reliable
  • Ideal for IHC/ICC/ISH and blots
  • Fluorescent
  • Heat Stable
  • Permanent mounting
  • Ideal for single and multiple labeling
  • One year expiry date
  • Stock solutions supplied in convenient dropper bottles promoting ease of handling
  • No wait times for mixing and dissolving powders or tablets
  • Sufficient reagents to produce 200 ml of working solution
  • Excitation: 645-685 nm*
  • Emission: peaks at 823 and 855 nm*

BCIP/NBT AP Substrate Kit forms an indigo, heat stable reaction product that can be dehydrated, cleared, and permanently mounted. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications. This substrate can also be used for developing blots or for In-situ Hybridization. At 20-30 minute development times, the sensitivity of this substrate is equivalent to the other alkaline phosphatase substrates. However, BCIP/NBT will continue to develop from several hours to overnight making it one of the most sensitive chromogenic substrates. This kit contains stock solutions in convenient dropper bottles.

Shipping Conditions:
Storage Conditions:
2-8 [o]C
Vector Laboratories
1 Kit