Ricinus Communis Agglutinin I (RCA I, RCA120), Agarose bound Citation Available Product Size(s) 2ml £242.00 AL-1083-2 Add to order Added to order 10ml £758.00 AL-1083-10 Add to order Added to order Academic Pricing Please contact us for academic pricing. Contact Us About this Product SKU: AL-1083 Additional Names: Lectins & Glycobiology Reagents [Affinity Binding Matrices ], Castor Bean Lectin, Galactose Binding lectin Application: Purification Conjugate: Agarose Extra Details: This lectin consists of two subunits of 60kDa which can be dissociated by reducing agents into closely related chains between 27kDa and 33kDa. One of the chains appears to be common to the B chain of another castor bean lectin, ricin, while the other chain is unique to RCA I. Agarose bound* Ricinus communis agglutinin I is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix. This coupling method provides several advantages over the traditional cyanogen bromide procedure: Maximum carbohydrate binding activity of the coupled lectins is retained Linkage is stable over a range of pH values Conjugated proteins are not leached off the beads by Tris or other routinely used buffers No residual charges are present after conjugation. This minimizes non-specific binding to the matrix. Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins. Inhibiting/Eluting Sugar: 200 mM galactose or lactose *4 mg lectin/ml gel Purification: Affinity Purified Research Area: Glycobiology Shipping Conditions: Ambient Storage Conditions: 2-8 [o]C DO NOT FREEZE Supplier: Vector Laboratories Manufacturer's Data Sheet:agarose-ricinus-communis-agglutinin Need Help? View product citations for antibodies AL-1083 on CiteAb